ISSN: 1659-2751



Establishment of a Protocol for Phase I, in vitro Multiplication of Bamboo (Guadua angustifolia)


There is a vast diversity of bamboo species throughout the world. In this project, phase I in vitro multiplication of Guadua angustifolia was investigated, applying modifications to already existing disinfection protocols to find the most suitable conditions to obtain of contaminant-free plants. Vegetative material was harvested from embryogenic, apical buds of mature plants and cut approximately 1 cm from each node. During the process, 17 different modifications on disinfection protocols were tested, most of which resulted in high levels of contamination. However, the solution with 2 g/L of benomyl and 2 g/L of streptomycin for 30 min, 2 % sodium hypochlorite for 30 min, 24 h incubation in sterile, distilled water followed by final 30 min disinfection in 2% sodium hypochlorite showed promising results. Different media supplemented with N-benzylaminopurine (BAP) were also evaluated at 0 mg/L, 1 mg/L, 3 mg/L and 5 mg/L, none of which induced budding in the explants. At 6 mg/L de BAP, however, budding was induced. The protocol generated in this study can be used as an alternative for in vitro multiplication of G. angustifolia.

Key words: disinfection, phase I, Guadua angustifolia, in vitro multiplication.

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