ISSN: 1659-2751

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Morphological Characterization and DNA Extraction from 11 Cassava Clones (Manihot esculenta Crantz) at EARTH University, Costa Rica

Abstract

Yuca (Manihot sculenta Crantz) is an important economic crop. Interest in this crop has increased, and institutions such as CIAT and IITA have begun to dedicate research to crop improvement through natural, conventional and biotechnological methods in order to develop new alternatives of increasing diversity and adaptation of this crop. Due to its variability in genotype and phenotype, a morphological characterization was carried out in the field in which the descriptors the leaf, stem and roots and 11 clones found at EARTH University were evaluated. A protocol of DNA extraction was adapted to evaluate fresh and freeze-dried tissues of the plant taking into account the conditions of the Natural Science Laboratory at EARTH University. It was determined that the descriptors that present the most variability between clones are: type of  central lobe, number of lobes per leaf, color of leafstalk, height of the plant, color of the stem, and diameter and longitude of the root. The protocol of extraction of CTAB-mini preparations -Harris 1996- in freeze-dried tissue produced a higher quantity of extracted DNA in electrophoresis in a 0,8 % agarose gel.

Key words: Manihot esculenta Crantz, DNA extraction, morphological characterization, descriptors.

Artículo en español (pdf) (1829)